With the advent of various molecular cloning technologies, such as the incorporation of DNA fragments and their transfer to bacteria in less than two hours, or the use of alternative gene cassettes, this branch of science has gained maximum flexibility and speed. In the near future, the disciplines of molecular cloning and biological synthetics will gain capabilities in the chemical manufacture of any specified DNA construct in vitro. These breakthroughs allow for speedier DNA structure and clone synthesis, as well as the development of gene therapy vectors, recombinant protein production techniques, and novel vaccinations.

In order to express recombinant proteins in bacteria, DNA fragments (open reading frames, ORFs) need to be inserted into an expression vector, routinely a plasmid, and this vector needs to be transferred into bacterial cells (transformation). The cells are then cultured and induced to produce recombinant proteins.

This online course will teach you how to use the microbial expression system (Bacteria: pET) for your recombinant protein expression. Learn about the bacterial manipulation to improve protein production.

This course will teach you about the basic principles of gene editing as well as how CRISPR-Cas9 works as a gene-editing technology. Throughout the eight weeks, you’ll learn about the major applications of CRISPR in the healthcare, agricultural, food, and energy industries.

You’ll get a deeper knowledge of the disruptive potential of biotech discoveries across numerous industries, as well as the capacity to explain your company’s biotech innovations to decision-makers.

All fields of biology have been touched by molecular cloning, which is the synthesis of recombinant DNA. In many molecular biology laboratories, gene cloning has become a widespread and standard approach.

The introduction of various molecular cloning technologies, such as integrating DNA fragments and transmitting them to bacteria in less than two hours, or the use of replaceable gene strains that can easily move between different structures, has given this branch of science the greatest flexibility and speed. In the near future, capabilities in the chemical synthesis of any specific DNA construct in vitro will develop in the disciplines of molecular cloning and synthetic biology. These advances enable faster production of DNA structures and clones, accelerate the development of gene therapy vectors, recombinant protein production processes, and new vaccines.

Real-time PCR can be used for both quantitative and qualitative analysis of nucleic acids in this field. Choosing the best method to use requires a comprehensive understanding of this technology. The Real-time PCR course will familiarise you with the technology’s basics, applications, implementation, analysis, and troubleshooting, and you will obtain great mastery and skill in applying this technique in your research and scientific path.

In this course, you will learn the basics of primer design, both manually and using the appropriate software. This course is presented in such a way that even if you do not have sufficient knowledge about it, after participating in this course and a little practice, you will be able to design your primers with sufficient mastery, use PCR for various purposes, and have the ability to debug PCR.