In this course, Unix is introduced to students as one of the most convenient tools for dealing with big data in the life sciences, including next-generation sequencing (NGS). NGS technologies produce enormous amounts of data each run that are difficult to cope with using GUI based tools, and raw files are difficult to open either. For this reason, sequencing data are produced and stored as text files so that they may be processed and handled easily. People who are skilled in bioinformatics know very well how to analyze data with PERL/Python programming language. This is very convenient, easy, and saves a lot of time.