Microbial systems have revolutionized biochemistry due to their ability to produce recombinant proteins. We are fast approaching the end of the days when a given protein could be purified with kilograms of plant or animal tissue or large amounts of biological fluid. In any new research project requiring purified proteins, researchers immediately consider recombinant forms of those proteins. It allows the biochemical characterization, use in industrial processes, and development of commercial products when a recombinant protein can be expressed and purified in large quantities.

In order to express recombinant proteins in bacteria, DNA fragments (open reading frames, ORFs) need to be inserted into an expression vector, routinely a plasmid, and this vector needs to be transferred into bacterial cells (transformation). The cells are then cultured and induced to produce recombinant proteins.

This online course will teach you how to use the microbial expression system (Bacteria: pET) for your recombinant protein expression. Learn about the bacterial manipulation to improve protein production.