Real time PCR

About Course

Techniques for detecting and amplifying nucleic acids are now extremely useful in biological research. These methods are used by researchers in a wide range of applications in the life sciences, including basic research, biotechnology, medicine, diagnostics, and more.

Real-time PCR can be used for both quantitative and qualitative analysis of nucleic acids in this field. Choosing the best method to use requires a comprehensive understanding of this technology. The Real-time PCR course will familiarize you with the technology’s basics, applications, implementation, analysis, and troubleshooting, and you will obtain great mastery and skill in applying this technique in your research and scientific path.

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What Will You Learn?

  • Understand the fundamentals of Real-time PCR and qRT-PCR
  • Able to design the primers and probed needed for a Real-time PCR reaction

Course Content

An introduction to PCR & Real-time PCR

How Real-time PCR works?

Detection using dyes and probes

Steps and components needed for conducting a Real-time PCR reaction

Real-time PCR data analysis

Assessment of curves , Ct and threshold

Primer and probe design

Optimization of Real-time PCR protocols

Normalization and quantification

Real-time PCR validation

Singleplex and multiplex experiments

Gene expression analysis