Courses – Nexintek

Real time PCR

NEXinTEK — Mon, 20 May 2019 04:32:14 +0000

Techniques for detecting and amplifying nucleic acids are now extremely useful in biological research. These methods are used by researchers in a wide range of applications in the life sciences, including basic research, biotechnology, medicine, diagnostics, and more.

Real-time PCR can be used for both quantitative and qualitative analysis of nucleic acids in this field. Choosing the best method to use requires a comprehensive understanding of this technology. The Real-time PCR course will familiarize you with the technology’s basics, applications, implementation, analysis, and troubleshooting, and you will obtain great mastery and skill in applying this technique in your research and scientific path.

Introduction to Primer Design and PCR

NEXinTEK — Fri, 17 May 2019 10:14:41 +0000

What will I learn: • Introduction to PCR • Needed Equipment and preparation in the laboratory • Reaction mixture components • Properties (length, GC content, Tm ...) of primers • Melting temperature, calculation method and related software • Secondary structures of primers, primer dimer and test method • Manual primer design using software under Gene Runner • How to obtain the desired DNA sequence from the NCBI • Specialized review of the existence of designed primers • Primer design with Primer 3 web application • Order primer synthesis, receive primers and prepare them for PCR • Preparing dNTP for PCR • Features DNA polymerases • Steps of performing PCR and setting the program of Thermal cycler device • Steps of horizontal electrophoresis and observation of PCR product on gel • PCR troubleshooting

Antibiotic discovery

NEXinTEK — Fri, 17 May 2019 09:57:10 +0000

The course will cover topics related to antimicrobial resistance including basic definitions and a review of the use of antimicrobials as well as the emergence and spread of resistance. It will guide you through the concepts as well as the importance of resistance dissemination. Through this course you will learn how bacteria become resistant to antibiotics and what mechanisms they may use to achieve this. As part of the course you will also receive training in antimicrobial susceptibility testing (AST) and the detection of specific resistance in a microbiological laboratory. It includes an additional module that guides you through genome analysis tools in order to detect resistance genes (and or any other genes of interest) in a simple and easy manner using freely available online tools.

Next-Generation Sequencing (NGS)

NEXinTEK — Fri, 17 May 2019 09:44:58 +0000

In this course, Unix is introduced to students as one of the most convenient tools for dealing with big data in the life sciences, including next-generation sequencing (NGS). NGS technologies produce enormous amounts of data each run that are difficult to cope with using GUI based tools, and raw files are difficult to open either. For this reason, sequencing data are produced and stored as text files so that they may be processed and handled easily. People who are skilled in bioinformatics know very well how to analyze data with PERL/Python programming language. This is very convenient, easy, and saves a lot of time.

Genome editing technology: CRISPR-Cas9

NEXinTEK — Fri, 17 May 2019 09:33:38 +0000

The CRISPR: Gene-Editing Applications online course provides an overview of the Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) gene-editing technology and its potential applications in a variety of industries.

This course will teach you about the basic principles of gene editing as well as how CRISPR-Cas9 works as a gene-editing technology. Throughout the eight weeks, you’ll learn about the major applications of CRISPR in the healthcare, agricultural, food, and energy industries.

You’ll get a deeper knowledge of the disruptive potential of biotech discoveries across numerous industries, as well as the capacity to explain your company’s biotech innovations to decision-makers.

Recombinant protein expression: From beginner

NEXinTEK — Fri, 17 May 2019 09:21:28 +0000

Microbial systems have revolutionized biochemistry due to their ability to produce recombinant proteins. We are fast approaching the end of the days when a given protein could be purified with kilograms of plant or animal tissue or large amounts of biological fluid. In any new research project requiring purified proteins, researchers immediately consider recombinant forms of those proteins. It allows the biochemical characterization, use in industrial processes, and development of commercial products when a recombinant protein can be expressed and purified in large quantities.

In order to express recombinant proteins in bacteria, DNA fragments (open reading frames, ORFs) need to be inserted into an expression vector, routinely a plasmid, and this vector needs to be transferred into bacterial cells (transformation). The cells are then cultured and induced to produce recombinant proteins.

This online course will teach you how to use the microbial expression system (Bacteria: pET) for your recombinant protein expression. Learn about the bacterial manipulation to improve protein production.

Vaccine design

NEXinTEK — Fri, 17 May 2019 09:09:00 +0000

In silico vaccine design, information and tools play a special but complementary role. In silico vaccine design may be a subset of emerging immunoinformatics. Immunoinformatics may be a link between immunology and bioinformatics. this system may be very effective in vaccine design, predicting system activity, testing for allergens, and drug design. The database is quite crucial in this strategy. Various bioinformatics software that can check information for vaccine design are known as in silico tools. Naturally, when the Corona virus appeared, you grasped the necessity of drug and vaccine design. Scientists in this discipline are hosting exhibits and scientific gatherings in most nations these days in order to develop effective chemical compounds to treat various infections.

Bioinformatics

NEXinTEK — Fri, 17 May 2019 08:56:59 +0000

Bioinformatics is the study of how to apply computer science, statistics, and probability in molecular biology. Bioinformatics is a branch of computer science that combines biology and computer science. The two fields have been brought together by advances in biology and new storage and retrieval technologies. Bioinformatics is an interdisciplinary field that uses tools from computer science, mathematics, genetics, chemistry, and physics to tackle numerous biological issues at the molecular level.

Techniques in Molecular cloning

NEXinTEK — Fri, 17 May 2019 08:04:33 +0000

The process of molecular cloning involves separating DNA sequences from any organism and placing them into a vector without altering their original sequence for amplification. Clones can be used to make large copies of DNA fragments that are intended for sequencing genes or expressing proteins to study protein function and expression. They can also be genetically altered in the laboratory to change their expression and function. Generating recombinant DNA, called Molecular Cloning, has affected all branches of biology. Gene cloning has become a standard procedure in a wide range of molecular biology laboratories.

With the advent of various molecular cloning technologies, such as the incorporation of DNA fragments and their transfer to bacteria in less than two hours, or the use of alternative gene cassettes, this branch of science has gained maximum flexibility and speed. In the near future, the disciplines of molecular cloning and biological synthetics will gain capabilities in the chemical manufacture of any specified DNA construct in vitro. These breakthroughs allow for speedier DNA structure and clone synthesis, as well as the development of gene therapy vectors, recombinant protein production techniques, and novel vaccinations.