Molecular genetics – Nexintek

Introduction to Primer Design and PCR

NEXinTEK — Fri, 17 May 2019 10:14:41 +0000

What will I learn: • Introduction to PCR • Needed Equipment and preparation in the laboratory • Reaction mixture components • Properties (length, GC content, Tm ...) of primers • Melting temperature, calculation method and related software • Secondary structures of primers, primer dimer and test method • Manual primer design using software under Gene Runner • How to obtain the desired DNA sequence from the NCBI • Specialized review of the existence of designed primers • Primer design with Primer 3 web application • Order primer synthesis, receive primers and prepare them for PCR • Preparing dNTP for PCR • Features DNA polymerases • Steps of performing PCR and setting the program of Thermal cycler device • Steps of horizontal electrophoresis and observation of PCR product on gel • PCR troubleshooting

Genome editing technology: CRISPR-Cas9

NEXinTEK — Fri, 17 May 2019 09:33:38 +0000

The CRISPR: Gene-Editing Applications online course provides an overview of the Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) gene-editing technology and its potential applications in a variety of industries.

This course will teach you about the basic principles of gene editing as well as how CRISPR-Cas9 works as a gene-editing technology. Throughout the eight weeks, you’ll learn about the major applications of CRISPR in the healthcare, agricultural, food, and energy industries.

You’ll get a deeper knowledge of the disruptive potential of biotech discoveries across numerous industries, as well as the capacity to explain your company’s biotech innovations to decision-makers.

Recombinant protein expression: From beginner

NEXinTEK — Fri, 17 May 2019 09:21:28 +0000

Microbial systems have revolutionized biochemistry due to their ability to produce recombinant proteins. We are fast approaching the end of the days when a given protein could be purified with kilograms of plant or animal tissue or large amounts of biological fluid. In any new research project requiring purified proteins, researchers immediately consider recombinant forms of those proteins. It allows the biochemical characterization, use in industrial processes, and development of commercial products when a recombinant protein can be expressed and purified in large quantities.

In order to express recombinant proteins in bacteria, DNA fragments (open reading frames, ORFs) need to be inserted into an expression vector, routinely a plasmid, and this vector needs to be transferred into bacterial cells (transformation). The cells are then cultured and induced to produce recombinant proteins.

This online course will teach you how to use the microbial expression system (Bacteria: pET) for your recombinant protein expression. Learn about the bacterial manipulation to improve protein production.

Vaccine design

NEXinTEK — Fri, 17 May 2019 09:09:00 +0000

In silico vaccine design, information and tools play a special but complementary role. In silico vaccine design may be a subset of emerging immunoinformatics. Immunoinformatics may be a link between immunology and bioinformatics. this system may be very effective in vaccine design, predicting system activity, testing for allergens, and drug design. The database is quite crucial in this strategy. Various bioinformatics software that can check information for vaccine design are known as in silico tools. Naturally, when the Corona virus appeared, you grasped the necessity of drug and vaccine design. Scientists in this discipline are hosting exhibits and scientific gatherings in most nations these days in order to develop effective chemical compounds to treat various infections.